Construction of human single-chain antibody gene for breast cancer
- VernacularTitle:人源乳腺癌单链抗体基因的构建
- Author:
Shubin TANG
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Scfv;
Construction
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objiective:To construct human single-chain antibody gene fragment for breast cancer.Methods:Total RNA was isolated from tumor adjacent lymphatic tissue of breast cancer patients.Then heavy and light chain(VH and VL) genes of antibody were amplified separately by RT-PCR,and assembled into ScFv genes by SOE-PCR.Results:The human single-chain antibody gene fragment for breast cancer was constructed and its size was about 750bp.Conclusion:The human single-chain antibody gene fragments were constructed successfully,which provides a way for constructing the human single-chain antibody library of anti-breast cancer.
