Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat
- VernacularTitle:大鼠脑组织蛋白质双向电泳技术的建立
- Author:
Chenglin ZHOU
- Publication Type:Journal Article
- Keywords:
Proteomics;
Two-dimensional electrophoresis;
Brain tissue;
Lysis buffer
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a two-dimensional electrophoresis technology on brain tissue protein of rat.Methods:Lysis buffer of different volume was taken to extract brain tissue proteins of rat,and different protein quantities(1mg,2mg,3mg) were taken to establish a two-dimensional electrophoresis.Coomassie brilliant blue was applied to stain protein,and patterns were analyzed.Results:With a molecular mass between 6.5~200ku and isoelectric points(pI) from 3~10,1mg proteins obtained 546 protein spots,2mg 780,and 3mg 805.Pattern of 2mg protein was the best.Conclusion:A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully.
