Study on hematopoietic reconstruction for bone marrow depression mice with the transplantation of mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide
- VernacularTitle:人参多糖动员的造血干/祖细胞移植重建造血衰竭小鼠造血功能的研究
- Author:
Hong WU
- Publication Type:Journal Article
- Keywords:
Ginseng polysaccharide;
Mobilization;
Hematopoietic stem/ progenitor cell;
Transplantation;
Hematopoietic reconstruction
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To study the hematopoietic reconstruction for bone marrow depression mice with mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide.Methods: Establish hematopoiesis failure model of mouse and transplant murine peripheral blood mononuclear cells mobilized by GPS.To observe the survival time,WBC,the weight of spleen,the number of CFU-S of recipient mice and chromosome chimerism.Results: After PBMNCs(1?105)mobilized by GPS from mice(male) were injected to the lethality irradiated mice,it can reduce the time of WBC recovery obviously;prolong the time of murine survival,increase the number of WBC of the recipient mice;it can also increase the number of CFU-S,the weight of spleen;Evaluating the Y chromosome by PCR,the result showed that 581bp Y chromosome sequence was examined in peripheral blood of the recipient mice.Conclusion: There are a lot of hematopoietic stem/ proginiter cells in peripheral blood of the mice mobilized by GPS,so it can obviously reconstruct the hematopoiesis of the bone marrow depression mice by transplantation.
