Construction of pcDNA3.1(+)/hFasL and its expression in COS-7 cells
- VernacularTitle:pcDNA3.1(+)/hFasL真核表达载体的构建及在COS-7细胞中的表达
- Author:
Xiujuan LI
- Publication Type:Journal Article
- Keywords:
FasL;
Eukaryotic expression;
COS-7 cell;
Plasmid
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the recombinant eukaryotic expression vector pcDNA3.1(+)/hFasL and investigate its transfection and transient expression in COS-7 cells.Methods:The full-length cDNA of hFasL was obtained from the plasmid vector pBluescript II KS(+)/hFasL cut with Xba I and then subcloned into eukaryotic expression vector pcDNA3.1(+).The recombinant plasmid pcDNA3.1(+)/hFasL was identified with restriction enzyme digestion and sequence analysis.It was then transfected into the COS-7 cells by Lipofectin Reagent and its expression was determined by immunocytochemical staining.Results:Restriction enzyme digestion analysis with XbaⅠ?DraⅡ?HindⅢ and DNA sequencing indicated the correct construction of the recombinant plasmid;Immunocytochemical staining showed the expession of hFasL on COS-7 cells.Conclusion:The construction and expression of the eukaryotic expression plasmid pcDNA3.1(+)/hFasL have been achieved successfully.
