Screening interactive proteins Mps1 by yeast two hybride system
- VernacularTitle:酵母双杂交系统筛选Mps1相互作用蛋白
- Author:
Qiong SHI
- Publication Type:Journal Article
- Keywords:
Yeast two-hybrid system;
Mps 1;
Protein interaction
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
objective:To screen and identify the interactive proteins of Mps1 and provide clues for the studies on Mps1 function on the chromosome segregation.Methods:The human embryonic cDNA library was screened with pDBLeu-Mps1 as bait plasmid by yeast two-hybrid system.And two Leu+ and LacZ+ positive yeast clones were obtained,which were contransformed to the MaV203 along with pDBLeu-Mps1 plasmid one to one by yeast simultaneous contransformation so as to identify the protein interactions again.The inserted fragments of identiyied positive clones were sequence and analyzed with BLAST in NCBI.Results: Only one positive clone identified one to one yeast simultaneous cotransformation,and the other was negative.The positive clone fragment was MAD1 protein by BLAST analysis in NCBI.Conclusion:To screen and initially identify the interactive protein of.Mps1 this research is a foundation for the study on the founctions of Mps1.
