The effect of NOS inhibitors on fetal rat neuron apoptosis during intrauterine distress and childhood's learning and memory
- VernacularTitle:一氧化氮合酶抑制剂对宫内窘迫胎鼠脑神经细胞凋亡和幼年学习记忆能力的影响
- Author:
Hua ZHANG
- Publication Type:Journal Article
- Keywords:
Intrauterine distress;
Fetal rat;
NOS inhibitors;
Apoptosis;
Learning ability
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Evaluating the effect of nitric oxide synthase(NOS) inhibitors on fetal rat neuron apoptosis during intrauterine distress and childhood's learning and memory.Methods:① Set up fetal rat intrauterine distress model.②Divide into the control group,reperfusion group,treatment group [injected N omega-nitro-L-arginine(L-NNA) 4mg/kg into pregnant rat's abdomen before ischemia and injected aminoguanidine(AG) 500mg/kg before operation],long-term observation group Ⅰ?Ⅱ?Ⅲ(the part of rats in control group,reperfusion group,treatment group were performed cesarean section on pregnant 19 days and bred to 40 day of age after delivery).③Measuring the content of cytochrome c in cytosol and examined the ability of learning and memory function.Results:①The content of cytochrome c in reperfusion group(6/12/24h) and treatment group(6/12/24h) were higher than those in the control group(P0.05).(3) Crossing platform times in long-term observation groupⅡ were fewer than those in long-term observation group Ⅰ?Ⅲ(P 0.05).Conclusion:NOS inhibitors play a obvious protective role in long-term intelligence of fetal rat after delivery through relieved neuron apoptosis during hypoxic ischemia encephalopathy.NOS inhibitors can be a valuable therapy method in intrauterine distress treatment.
