Effect of salvia miltiorrhiza bunge on proliferation and differentiation of osteoblasts in rats
- VernacularTitle:丹参对大鼠成骨细胞增殖和分化的影响
- Author:
Guoliang LIU
- Publication Type:Journal Article
- Keywords:
Salvia Miltiorrhiza Bunge;
Cell culture;
Osteoblasts
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Salvia Miltiorrhiza Bunge on cell proliferation and differentiation of osteoblasts in rats cultured in vitro.Methods:Osteoblasts from cranium of newborn SD rats were cultured by collagenase method.MTT was used to determine the proliferation of cultured osteoblasts affected by different concentrations of Salvia Miltiorrhiza Bunge at different time points(24,48,72 hrs after medication).Differentiating effect of different concentrations of Salvia Miltiorrhiza Bunge in the cell was evaluated through the examination of alkali phosphate(ALP) activity.Results:0.05%,0.10%,0.15% of Salvia Miltiorrhiza Bunge could enhance the cell proliferation rate(P
