Determination of ginsenoside Rg1 and notoginsenoside R1 in Yiqi Gushen Dialysis Liquid by solid phase extraction and TLC densitometric method
- VernacularTitle:固相萃取薄层扫描法测定益气固肾透析液中三七皂苷R1和人参皂苷Rg1的含量
- Author:
Ruizhi ZHAO
;
Daixin ZHAO
;
Nizhi YANG
- Publication Type:Journal Article
- Keywords:
ginsenoside Rg1;
notoginsenoside R1;
Yiqi Gushen Dialysis Liquid;
determination;
TLCS
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the method of determining ginsenoside Rg1 and notoginsenoside R1,and control the quality of Yiqi Gushen Dialysis Liquid(Radix et Rhizoma Notoginseng,Radix Astragali,etc.) METHODS: Sample was pretreated with solid phase extraction using ODS C_13 cartridges.Ginsenoside Rg1 and Notoginsenoside R1 were separated with TLC method.The mobile phase was chloroform-methanol-water(3∶0.8∶0.1),developed twice.The coloration was 10% sulphuric acid ethanol solution.The detection wavelength was 560 nm. RESULTS: The calibration curves for ginsenoside Rg1 and notoginsenoside R1 were A=113.4 C-34.976,(r=0.997),A=92.14 C-13.597,(r=0.997),respectively,the linear ranges were between 2.64-13.2 mg and 0.85-8.5 mg,and the detection limit was 0.88 mg and 0.65 mg,respectively.The average recoveries were 100.31% and 98.72%,respectively.The contents of the sample were between 5.683-5.764mg,1.528-1.599 mg per 10 mL,respectively. CONCLUSION: The proposed method for estimating ginsenoside Rg1 and notoginsenoside R1 is simple,precise,specific,sensitive and accurate and can be used for quality control of Yiqi Gushen Dialysis Liquid.
