Determination of free and hydrolysable gallic acid in Acer Truncatum Bunge by HPLC
- VernacularTitle:HPLC测定元宝枫中游离和水解没食子酸的含量
- Author:
Jun LI
;
Jinlong LI
;
Yaonan WANG
;
Yingxia ZHANG
- Publication Type:Journal Article
- Keywords:
Acer Truncatum Bunge;
gallic acid;
high performance liquid chromatography
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect free and hydrolysable gallic acid in Acer Truncatum Bunge by HPLC. METHODS: A method for determining gallic acid in leaves of Acer Truncatum Bunge.The operating conditions HPLC were Diamonsil C_18 column(5 ?m,4.6 mm?250 mm) at room temperature,methanol-0.025 mol/L phosphoric acid solution(15∶85) as mobile phase at a flow of 1.0 mL/min and UV detection at 272 nm. RESULTS: The contents of free and hydrolysable gallic acid were 0.045% and 1.546%,repectively.The relative standard deviations of free and hydrolysable gallic acid were 1.06% and 2.43%,respectively. CONCLUSION: The contents of free and hydrolysable gallic acid in Acer Truncatum Bunge in different periods are different.
