Induced Cardiomyogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Vitro
- VernacularTitle:体外诱导骨髓间质干细胞向心肌细胞的分化
- Author:
Yan HUANG
;
Yinghui LI
;
Fen YANG
;
Zhongquan DAI
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells;
cardiomyogenic differentiation;
5-azacytidine;
Immunohistochemistry;
RT-PCR
- From:Space Medicine & Medical Engineering
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 5-azacytidine on cardiomyogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Method BMSCs were isolated from the marrow of adult SD rat’s femoral /tibial bones.Different concentration of 5-azacytidine were added to primary BMSCs on 3 d and cultured for different times.Cardiomyogenic differentiation of BMSCs was observed by immunohistochemistry and RT-PCR. Result After treated with 5-azacytidine, BMSCs proliferated slowly, became spindle-shaped after 10 d and aligned in a striated pattern after 20 d.TnT positive cells were showed by Immunohistochemistry and they expressed two cardiac-marked genes GATA-4 and ?-MHC.Thus 5-azacytidine induced cardiomyogenic differentiation of BMSCs in a time and concentration-dependent manner. Conclusion Our study suggests that bone marrow mesenchymal stem cells can differentiate into cardiomyocytes in vitro. They are ideal donor cells in cellular cardiomyoplasty for treatment of myocardial infarction.
