Responses of Cultured Neuronal Network to Electric Pulses Stimuli with Different Intervals
- VernacularTitle:培养神经元网络对不同间隔电脉冲刺激的响应
- Author:
Chuanping CHEN
;
Lin CHEN
;
Yunsheng LIN
;
Shaoqun ZENG
- Publication Type:Journal Article
- Keywords:
cultured neuronal network;
multi-electrode arrays;
electrical stimuli;
evoked response;
synaptic modification
- From:Space Medicine & Medical Engineering
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the activities of cultured neuronal network in response to electric pulses stimuli with different intervals. Method Pairs of electric pulses with different intervals were added to rat hippocampal neurons cultured on multi-electrode arrays (MEA) substrate. Result The second response duration of the network increased with the decrease of the interval between two pulses. It was typical that the second duration after pulses with 10 ms interval increased greatly and it could be divided into two stages, i.e. quick response (within 10 ms) and slow response (near or beyond 100 ms). However, following pulses with 100 ms interval,there is practically no relation between the two responses; the second response duration was shorter and the number of spikes was fewer and their amplitudes were lower, which is similar to the result of single electric pulse stimulus. Conclusion The activity of cultured neuronal network was different in response to different electric stimulation patterns. Compared with spontaneous firing, the activity of the network is potentiated or depressed respectively after different stimulations.
