Sequence and analysis of Cyt b gene of Agkistrodon and its adulterants sold in market
- VernacularTitle:蕲蛇药材及其市售混淆品的Cyt b基因序列与分析
- Author:
Wencheng SONG
;
Shewu SONG
;
Daofang LIU
;
Haibing LIU
;
Lihua SONG
- Publication Type:Journal Article
- Keywords:
Agkistrodon;
Cyt b gene;
molecular identification
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To differentiate the sequences of Cyt b gene Agkistrodon from its adulterants sold in the market in order to provide molecular evidence for identification of Agkistrodon. Methods Cyt b gene was used to sequence and analyze Agkistrodon and its adulterants sold in the market. Results There was a mixed phenomenon in Agkistrodon species. The differences of Cyt b gene sequence between Agkistrodon and its adulterants sold in the market are significant: the difference rates among the species of Agkistrodon are 0%—0.91%, the difference rates between Agkistrodon and its adulterants are 18.57%—23.78%. Conclusion The characteristics of Cyt b gene sequence can be used as a better molecular marker for authenticating Agkistrodon from its adulterants.