THE ACTIVATION OF EXTRACELLULAR SIGNAL-REGULATED KINASE AND cAMP RESPONSIVE ELEMENT BINDING PROTEIN IN THE ANTERIOR CINGULTE CORTEX INDUCED BY PERIPHERAL INJECTION OF FORMALIN
- VernacularTitle:外周注射福尔马林诱导大鼠前扣带皮层中ERK-CREB的激活
- Author:
Yongjing GAO
- Publication Type:Journal Article
- Keywords:
Anterior cingulate cortex;
Extracellular signal-regulated kinase;
cAMP responsive element binding protein;
Pain;
Immunohistochemistry;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the activation of extracellular signal-regulated kinase(ERK) and cAMP-responsive element binding protein(CREB) in the anterior cingulate cortex(ACC) induced by peripheral injection of formalin.Methods 5% formalin was injected subcutaneously into the unilateral hindpaw.The rats were perfused or killed and the ACC dissected at different time points.The activation of phosphorylated ERK(pERK) and phosphorylated CREB(pCREB) in the ACC were observed by immunohistochemical and Western blotting techniques.Results Subcutaneous injection of formalin induced the activation of pCREB and pERK in the ACC.Formalin-induced the activation of pERK and pCREB in the ACC peaked at 3 min and 30 min after stimulation,respectively.The total ERK and CREB had no significant changes.Conclusion The ACC could receive peripheral noxious information and the ERK and CREB in the ACC might involve in the processing of the primary unpleasentness of pain.
