ESTABLISHMENT OF AN IMMORTALIZED RAT ASTROCYTE STRAIN EXPRESSING ENKEPHALIN REGULATED BY DOXYCYCLINE AND ITS ANALGESIC EFFECT ON RAT CHRONIC NEUROPATHIC PAIN
- VernacularTitle:强力霉素诱导表达脑啡肽细胞株的构建及其对慢性神经痛大鼠的镇痛作用
- Author:
Ying XU
;
Yuke TIAN
- Publication Type:Journal Article
- Keywords:
Human preproenkephalin gene;
Chronic pain;
Astrocyte;
Retrovirus infection method;
Cell transplantation;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an immortalized rat astrocyte strain(IAST) expressing enkephalin regulated by doxycycline(Dox) and observe its analysesic effect on rat chronic neuropathic pain. Methods Retrovirus infection method was employed to develop an immortalized rat astrocyte strain expression enkephalin regulated by doxycycline.hPPE gene expression level of IAST/Tet-On/hPPE strain was detected by Real time-PCR and radioimmunoassay.Its analgesic potential was investigated by mechanical paw withdrawal thresholds after these cells were implanted into the subarachnoid space of chronic constrictive injury(CCI) rats.The expression of Fos protein in the dorsal horn of spinal cord was determined by immunohistochemistry. Results An immortalized rat astrocyte strain secreting enkephalin under the control of doxycycline was established successfully.After transplantation of IAST/Tet-On/hPPE cell into the subarachnoid space of chronic constrictive injury(CCI) rats, the sensitivity of mechanical allodynia and the expression of Fos protein were significantly decreased(P
