COMBINATION OF GOREMOR VESSEL ELECTROACUPUNCTURE AND TRANSPLANTED NEURAL STEM CELLS PROMOTES INJURED SPINAL CORD TISSUE PRODUCTING NERVE GROWTH ACTIVE SUBSTANCES
- VernacularTitle:督脉电针与神经干细胞移植联合应用促进大鼠受损伤脊髓组织产生神经生长活性物质
- Author:
Xiaobin LI
- Publication Type:Journal Article
- Keywords:
Goremor vessel electroacupuncture;
Neural stem cells transplantation;
Spinal cord injury;
Cyclic adenosine-monophosphate;
Neural growth active substances;
Radioimmunoassay;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether combination of Goremor vessel electroacupuncture and transplanted neural stem cells could promote the completely transected spinal cord tissue producting some nerve growth active substances. Methods Fourty-five adult rats were divided into normal group,control group,neural stem cells transplanted group(NSCs group),Goremor vessel electroacupunctural group(EC group) and Goremor vessel electroacupuncture plus NSCs transplanted group(EC+NSCs group).All animals(except for the normal group) were performed complete transection on T10 segment of spinal cord.The electroacupunctural treatment started at the fifth day after operation among EC group and EC+NSCs group.The sacrifice time of rats was the 14d and 28d after operation respectively.The cyclic adenosine-monophosphate(cAMP) radioimmunoassay and Western blotting test for levels of Neuroltrophin-3(NT-3),neurotrophic factor receptor——Trk and growth associated protein-43(GAP-43) were made in the tissue of injured spinal cord. Results 1.Fourteen days after operation,cAMP concentration at cranial segment and caudal segment of injured spinal cord segment was lower in all groups than that of normal group,except for EC group.cAMP concentration at injured segment was increased slightly in EC group and EC+NSCs group than that of normal group,Twenty-eight days after operation,cAMP concentration at cranial segment and caudal segment of injured spinal cord segment maintained a higher level in EC+NSCs group.cAMP concentration at injured segment was increased slightly in NSCs group and EC+NSCs group than normal group.2.The expression of NT-3,Trk and GAP-43 was more prominent in EC group and EC+NSCs group.Meanwhile,the expression of three proteins was lower in control group and NSCs group than that of EC group and EC+NSCs group.Conclusion Goremor vessel electroacupuncture may promote the injured spinal cord tissue producting cAMP.The combination of Goremor vessel electroacupuncture and transplanted neural stem cells may maintain higher cAMP concentration and tend to increase the concentration of NT-3,Trk and GAP-43 in injured spinal cord tissue.
