EXPRESSIONS OF IL-1? AND IL-1RI mRNA IN THE RAT CAROTID BODY
- VernacularTitle:白细胞介素-1?及其Ⅰ型受体mRNA在大鼠颈动脉体中的表达
- Author:
Xi WANG
;
Xijing ZHANG
;
Xin LI
;
Xiaoli DUAN
;
Gaili LI
;
Gong JU
;
Bairen WANG
- Publication Type:Journal Article
- Keywords:
IL-1?;
IL-1RI mRNA;
Carotid body;
In situ hybridization;
Immunofluorescence;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressions of IL-1 receptor type Ⅰ(IL-1RI) mRNA and IL-1? protein in the rat carotid body. Methods In situ hybridization,immunofluorescence double staining and Western blotting methods were used. Results The result of in situ hybridization showed that the positive signal of IL-1? mRNA was mainly located in the glomus cells of the carotid body.The result of immunofluorescence double staining showed that IL-1? protein also expressed in the glomus cells of the organ.The Western blotting proved that the IL-1? immunoreactive band appeared at 18kD,consistent with the molecular weight of the cytokine.Conclusion The glomus cells of the rat carotid body not only express IL-1RI mRNA,but also IL-1?.
