The effects of red laser irradiation on endothelial cells secreting and expressing nitric oxide
- VernacularTitle:红光激光对内皮细胞一氧化氮分泌及表达的影响
- Author:
Wenjian LI
;
Yanan WANG
;
Yunliang GUO
;
Bingxia SHI
- Publication Type:Journal Article
- Keywords:
Lasers;
Endothelial cells;
Nitric oxide;
Nitric oxide synthesis
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe endothelial cells secreting and expressing nitric oxide by using red laser irradiation ( RLI) on cultured endothelial cells. Methods Cultured endothelial cells were irradiated with a red laser for 10, 20, and 30 minutes, respectively, and the concentration of nitric oxide in the cell supernatant was measured after 15 min,30 min.1 h,3 h and 6 h. In addition, the expression of inducible nitric oxide synthetase (iNOS) and endotlielial nitric oxide synthetase (eNOS) was measured through immunohistochemical staining. Results Compared with that in the control group, the expression of eNOS by the endothelial cells and the concentration of NO were stimulated by 2 mW RLI for 10 min, 20 min and 30 min, and reached a peak at 1 h, then declined gradually. The expression of iNOS, however, showed no significant difference. Conclusion RLI can increase NO concentrations in endothelial cells by stimulating the expression of eNOS rather than iNOS.
