The fingerprints of Fructus Gardeniae by HPCE
- VernacularTitle:栀子的毛细管电泳指纹图谱研究
- Author:
Zhifei HOU
;
Guoxiang SUN
;
Weifen LIU
- Publication Type:Journal Article
- Keywords:
Fructus Gardeniae;
capillary electrophoresis;
fingerprints;
information index;
relative information amount index
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: A capillary electrophoresis fingerprints(CEFP) method of Fructus Gardeniae was established to evaluate its quality. METHODS: The background electrolyte(BGE) was 25 mmol/L sodium borate solution containing 10% acetonitrile.The detection wavelength was 228 nm and 24 kV was applied.Fructus Gardeniae was extracted by water and injectded for 15 s(9 cm).Some parameters were used to evaluate the similarities.(RESULTS): 24 co-possessing peaks were selected as the fingerprint peaks of Fructus Gardeniae taking chlorogenic acid peak as the reference peak.The similarities between each of the ten places and the standard CEFP of Fructus Gardeniae were evaluated both qualitatively and quantitatively.The CEFP was also evaluated by the information index(I) and the relative information index(I_r). CONCLUSIONS: The CEFP has acceptable precision,reproducibility and can be used to control the quality of Fructus Gardeniae.
