Determination of notoginsenoside R_1,ginsenoside Rg_1,Rb_1 in Naomaitai Capsules by HPLC
- VernacularTitle:HPLC同时测定脑脉泰胶囊中三七皂苷R_1与人参皂苷Rg_1、Rb_1的含量
- Author:
Yang JIN
- Publication Type:Journal Article
- Keywords:
Naomaitai Capsules;
HPLC;
notoginsenoside R_1;
ginsenoside Rg_1、Rb_1
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method of determining notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 in Naomaitai Capsules(Radix et Rhizoma Ginseng Rubra,Radix et Rhizoma Notoginseng,Radix Angelicae Sinensis,etc.) by HPLC. METHODS: Notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 were determined by HPLC gradient elution method,Inertsil-ODS-3(5 ?m 4.6 mm?250 mm) column with Acetonitrile-water as the mobile phase was used for gradient elution.The flow rate was at 1.0 mL/min and the detective wavelength was at 203 nm.The column temperature was at 35 ℃. RESULTS: There was a good linear relationship at a range of 0.205-1.539 ?g for notoginsenoside R_1(r=0.999 6),the average recovery of 98.07%, and RSD of 0.51%(n=5),0.838-6.285 ?g for ginsenoside Rg_1(r=0.999 4),the average recovery of 97.66%,and RSD of(0.53%)(n=5),0.822-6.165 ?g for ginsenoside Rb_1(r=0.999 6),the average recovery of 98.19%,and RSD of 0.44%(n=5). CONCLUSION: The method is simple,reproducible and accurate with a strong specificify,and can be used for the quality control of Naomaitai Capsules.