Effect of Hushengujingfang on the Proliferation of Rat Mesangial Cells
- VernacularTitle:护肾固精方对大鼠系膜细胞增殖周期的影响
- Author:
Qingfa ZHOU
;
Yang GAO
;
Hongyan WU
- Publication Type:Journal Article
- Keywords:
Hushengujingfang;
mesangial cell;
multiplication cycle;
apoptosis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the Hushengujingfang (HSGJF), a traditional Chinese prescription, on the biological behaviour of rat mesangial cell (MC). Methods MC was cultured in vitro, and the serologic pharmacology method was employed. The cells were divided into serum groups of HSGJF, LPS group and control group. Added the different concentration actors to MC and cocultured then made into unicell suspension. After examining it by flow cytometry, the percentage of G 0 /G 1 period, S period, G 2 /M period in cell cycle and the inhibition percentage of S period and cell proliferation index were calculated. Result The actors of different concentration had different effects to the multiplication cycle of MC and delayed the entry of cell cycle at G 0 /G 1 period. HSGJF induced apoptosis of MC. Conclusion One of action mechanism of HSGJF treating mesangial proliferative nephritis and other renal glomerular diseases is to inhibit MC proliferation and promote apoptosis.
