RP-HPLC Determination of the Content of Ephedrine?HCl in Xiaoyan Zhike Plaster
- VernacularTitle:RP-HPLC法测定消炎止咳贴中盐酸麻黄碱的含量
- Author:
Ming QIAO
;
Jianwei HAN
;
Min FU
- Publication Type:Journal Article
- Keywords:
Xiaoyan Zhike Plaster;
ephedrine?HCl;
RP-HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method to determine the content of ephedrine?HCl in Xiaoyan Zhike Plaster. Method The sample was prepared according to 2005 edition of Chinese Pharmacopocia, the column of Inertsil-3 ODS (4.6 mm?250 mm, 5 ?m) was used. The mobile phase was composed of methanol-0.1% of phosphoric acid (5∶95). The flow rate was 1 mL/min, with UV detection at 207 nm. Results The linear range was 0.041～0.41 ?g (r=0.999 8). The lowest limit of detection was 0.025 6 ?g. The average recovery and the precision was 99.55% and 2.58%. Conclusion The method is simple, sensitive and accurate, and can be used for the quality control of Xiaoyan Zhike Plaster.
