Determination of the Content of Ephedrine Hydrochloride in Wenyang Tongbi Wan by RP-HPLC
- VernacularTitle:RP-HPLC法测定温阳通痹丸中盐酸麻黄碱的含量
- Author:
Zhuangsong ZHU
;
Jianchang LIN
;
Zhimei WANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Wenyang Tongbi Wan;
ephedrine hydrochloride;
content
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of the content of ephedrine hydrochloride in Wenyang Tongbi Wan. Methods A DDS-C18 column (4.6 mm?150 mm, 5 ?m) was used. The mobile phase was methane-water (1∶1). The column temperature was 30 ℃. The flow rate was 1 mL/min. Results There was good linearity of ephedrine hydrochloride in the range of 1.308 6～3.918 0 ?g (r=0.999 3, n =5). The average recovery was 98.91% (RSD=0.68%, n =5). Conclusion This method is simple, accurate and repeatable, and can be used for quality control of Wenyang Tongbi Wan.
