Optimum culture media and liquid conditions in shaking flasks for laccase production by Ganoderma lucidum
- VernacularTitle:灵芝液体发酵产漆酶最佳培养基和培养条件研究
- Author:
Ying XU
;
Jin LAN
- Publication Type:Journal Article
- Keywords:
Ganoderma lucidum (Leyss. ex Fr.) Karst;
laccase;
culture media;
culture condition
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the optimum culture media and liquid conditions in shaking flasks for laccase production by Ganoderma lucidum. Methods Taking S_3 stain of G. lucidum as test materials and laccase activity of G. lucidum as measurement index to optimize the culture media and liquid conditions through orthogonal test. Results The optimum culture components of media were: Glucose 30 g/L, cotton 0.2%, (NH_4)_2HPO_4 0.66 g/L, casein 0.5%, Tween-80 0.15 mL; the optimum conditions were: initial pH value of medium was 5.5, 75 mL medium was in 250 mL-flask, inoculation was 12.5%, mycelium age was 5?24 h for 9?24 h culture. Conclusion Laccase activity of G. lucidum has been improved remarkably in the optimum culture media and liquid conditions.
