Effects of urotensin Ⅱ receptor antagonist on the level of urotensin Ⅱin plasma and bronchoalveolar lavage fluid in rats with acute lung injury
- VernacularTitle:尾加压素Ⅱ受体拮抗剂对急性肺损伤大鼠血浆及肺泡灌洗液中尾加压素Ⅱ的影响
- Author:
Rongde LAI
;
Zijing LIANG
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Plasma;
Bronchoalveolar lavage fluid;
UrotensinⅡ;
UrotensinⅡ receptor antagonist
- From:
Chinese Journal of Emergency Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of urotensinⅡ(UⅡ)receptor antagonist(URA)on UⅡ in plasma and bronchoalveolar lavage fluid(BALF)in rats with acute lung injury(ALI).Methods Twenty eight Sprague Dawley(SD)rats were randomly divided into four groups:saline control group(group A),the other three groups(group B,group C,group D).Rats were injected with oleic acid and URA to produce ALI models.Three hours after injection,arterial blood was drawn for blood gas analysis in all rats.Immediately after this,blood of heart was collected in group A and group B.Then blood of heart were collected after 12 hours in group C and after 24 hours in group D.Rats were killed when blood of heart was drawn.Bronchoalveolar lavage(BAL)with saline was performed in the right lung.BALF was centrifugated and the upper fluid was put to the EP tube.Plasma was separated from the blood of heart.Both plasma and BALF were kept at -80 ℃ to be determined.Results Compared with group A,arterial pressure of oxygen was significantly decreased in the group B,group C,group D(P
