Effect of Yifa Compound on the Growth of Hair Papilla Cells
- VernacularTitle:益发复方对毛乳头细胞生长的影响
- Author:
Shengli CHEN
;
Dacan CHEN
- Publication Type:Journal Article
- Keywords:
ALOPECIA/TCD therapy;
SEROPHARMACOLOGY;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To investigate the effect of serum containing Yifa Compound(YC) on the growth of hair papilla cells.【Methods】Hair papilla cells were cultured in vitro.After subculture for 4～6 generations,hair papilla cells at logarithmic growth phase were cultured with YC-containing serum(at the concentrations of 1.25,2.5 and 5.0?g/L respectively) and blank serum respectively.After the culturing,the proliferation curve of hair papilla cells was drafted by methylthiazolyltetrazolium(MTT) assay and their growth was observed under scanning electron microscope.【Results】Optical density of hair papilla cells was higher at logarithmic growth phase while lower at growth lag phase in high-dose YC-containing serum group than that in blank serum group.Moderate-and high-dose YC-containing serum promoted the agglutinating radial growth.【Conclusion】Serum containing YC can promote the proliferation of hair papilla cells.
