Establishment of HBV-Marker-Producing Nude Mice Model of Human Transplanted Liver Cancer
- VernacularTitle:分泌乙型肝炎病毒标志物的裸鼠人肝癌移植瘤模型的建立
- Author:
Yuhui LU
;
Linchun FU
;
Haiyan HU
- Publication Type:Journal Article
- Keywords:
NEOPLASMS,EXPERIMENTAL;
TUMOR VIRUS INFECTIONS;
HEPATITIS B VIRUS;
DISEASE MODELS,ANIMAL
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To establish a HBV-marker-producing nude mice model of human transplanted liver cancer(HTLC).【Methods】2.2.15 cells,a HePG2 cell line transfected with HBV genome,were injected subcutaneously into athymic BALB/c-nu mice aged 4～6 weeks.The growth of tumor was observed every week.After 36 days the nude mice were executed and HBV markers of HBsAg,HBeAg,and HBV-DNA as well as alpha-fetoprotein(AFP)in serum were detected.The pathological features of tumor tissues were examined under light microscope.【Results】The incidence of HTLC and the time of HTLC appearing were correlated with the number of transplanted 2.2.15 cells.Serum HBsAg,HBeAg,and HBV-DNA were positive in the HTLC transplanted mice,the serum content of AFP was(350?11.5) ?g/L,and pathological changes under light microscope were similar to human,indicating that the model has some similar histological characteristics of the human liver cancer infected by HBV.【Conclusion】The nude mice model of human transplanted liver cancer,which can produce HBV markers,is established successfully in this study.
