Application of a bidirectional amplification of specific alleles polymerase chain reaction technique in study of SNPs
- VernacularTitle:双向等位特异PCR技术及其在单核苷酸多态性研究中的应用
- Author:
Chenhui ZHOU
- Publication Type:Journal Article
- Keywords:
Sinlgle nucleotide polymorphism;
Allelic specific bidirectional amplification of specific alleles polymerase chain reaction;
PTEN
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a bidirectional amplification of specific alleles polymerase chain reaction(Bi-PASA PCR)technique system and to apply this technique to study of single nucleotide polymorphism(SNP)of genes.Methods: The information of nucleotide sequences and their corresponding SNPs of gene were obtained from genBank of National Central of Biotechnology Information.The primers were designed by Primer 5.0 software and their specificity was tested by NCBI Blast 2.0 software.Results: They successfully designed and established the technique system of Bidirectional amplification of specific alleles Bi-PASA.The method were applied to study SNPs of PTEN(phosphatase and tensin homology deleted on chromosome ten,PTEN).The superiority and stability of Bi-PASA PCR technique on determining SNPs of genes have been tested.Conclusions:The Bi-PASA PCR technique is a new method for determination of SNP with more convenience,more specificity,less cost and easy availably and has much more advantage especially in the study of population gene polymorphism.
