Prepartion of reference substance of flavonoid in Desmodium styracifolium by RP-HPLC
- VernacularTitle:反相高效液相色谱法制备广金钱草黄酮对照品
- Author:
Guoguang WANG
;
Yanwei LU
;
Surong ZHAO
;
Chutse WU
- Publication Type:Journal Article
- Keywords:
Desmodium styracifolium;
reference substance;
vicenin-2
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop an RP-HPLC method for preparing the reference substanc of vicenin-2 in Desmodium styracifolium. METHODS: Ethanol-extract of desmodium styracifolium was isolated and purified by RP-HPLC combining solvent extraction with column chromatography and recrystalliztion.The purity and content of vicenin-2 were identified by HPLC. RESULTS: The flvonoids were completely separated under this chromatographic condition.The purity of the reference substance was 99.0% or above. CONCLUSION: The method is simple,accurate,better on repeatability,and effective to yield high-purity product.It can be used as reference substance for the research of herbal medicine.
