Determination of 2 caffeic acid derivatives in Echinacea extracts by RP-HPLC
- VernacularTitle:RP-HPLC测定紫锥菊提取物中2种咖啡酸衍生物的含量
- Author:
Du XU
;
Yuwei TANG
;
Wei ZHU
- Publication Type:Journal Article
- Keywords:
Echinacea purpurea root;
HPLC;
caftaric acid;
chicoric acid
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To estahlish a RP-HPLC method of determining two caffeic acid derivatives in Echinacea extract and to determine 5 Echinacea purpurea root extracts. METHODS: RP-HPLC was applied to determine 2 caffeic acid derivatives in E.purpurea extract:caftaric acid and chicoric acid.HPLC conditions were as follows: Agilent ZORBAX StableBond C_(18) column(5 ?m,4.6 mm?250 mm) was used,with A(acetonitrile) —B(0.1% H_3PO_4) gradient elution as a mobile phase.The flow rate was 1.2 mL/min.The detection wavelenth was set at 330 nm. RESULTS: 2 caffeic acid derivatives were separated well.The linear range for caftaric acid and chicoric acid were 0.064-0.416 ?g and 0.1-0.7 ?g,respectively.Its average recoveries were 99.37% with RSD of(1.50%) and 100.44% with RSD of 1.71%,respectively. CONCLUSION: The method is simple,accurate,strong specificity and can be used to control the quality of E.purpurea extract and new medicine development.
