Effects of Tail Suspension on Renin-angiotensin System in Rat Myocardium
- VernacularTitle:尾吊大鼠心肌组织局部肾素-血管紧张素系统的表达变化
- Author:
Junxiang BAO
;
Lifan ZHANG
;
Jin MA
- Publication Type:Journal Article
- Keywords:
weightlessness simulation;
myocardium;
renin-angiotensin system;
gene expression
- From:Space Medicine & Medical Engineering
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of gene and protein expressions of key components of renin-angiotensin system (RAS) in myocardium of simulated weightless rats. Method Tail-suspended rats were adopted as animal model of simulated weightlessness. RT-PCR and Western blot analysis were performed to examine mRNA and protein expressions in rat myocardium, respectively. Result After 1 week tail-suspension,no obvious changes in angiotensinogen (AGT), angiotensin converting enzyme (ACE), 1a and 1b subtype of type 1 angiotensin Ⅱ receptor (AT 1aand AT 1b), key components of RAS in myocardium were observed. But after 4 weeks tail-suspension, the expressions of AGT mRNA and higher molecular weight band of AGT protein increased significantly (P
