Effect of Polysaccharides-2b from Mudan Cortex on High Glucose Induced Proliferation of Rat Mesangial Cells and Reactive Oxygen Species in Culture
- VernacularTitle:牡丹皮多糖2b对高糖所致大鼠系膜细胞增生及活性氧产生的影响
- Author:
Bo YANG
;
Yeshou SHEN
;
Zhiping ZHAO
- Publication Type:Journal Article
- Keywords:
polysaccharides-2b from mudan cortex;
mesangial cells;
high glucose;
reactive oxygen species
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of high glucose on proliferation of rat mesangial cells and reactive oxygen species (ROS) in culture and the intervention of polysaccharides-2b from Mudan Cortex (PSM2b). Methods Cell proliferation was assessed by MTT colorimetric assay. Colorimetric assay was used to detect SOD and MDA in the supernatant of high glucose culture of rat mesangial cells. Results PSM2b could significantly inhibit the proliferation of mesangial cells, the inhibiting effect showed a dose-dependent manner. And SOD increased with PSM2b compared with that only with high glucose, while MDA decreased. Conclusion High glucose contributes to an increase in rat mesangial cells and ROS produced by rat mesangial cells and PSM2b can better control high glucose-induced proliferation of rat mesangial cells and oxidative stress.
