Content Determination of Polysaccharides in Radix and Rootlet Ophiopogonis by the method of Phenylhydrate Sulfuric Acid
- VernacularTitle:苯酚-硫酸法测定麦冬须根中多糖的含量
- Author:
Jiaqi HE
;
Zhensheng YAO
;
Yaokang XIONG
- Publication Type:Journal Article
- Keywords:
Radix Ophiopogonis;
Rootlet Ophiopogonis;
polysaccharides;
spectrophotometey
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the content of polysaccharides in Radix and Rootlet ophiopogonins. Methods Phenylhydrate-sulfuric acid method was used. Results The maximum absorption wavelength was 485 nm. Linear range was 0.101～1.012 mg/L (A=0.826 7C+0.016 3, r=0.999 2). The polysaccharide content in Radix ophiopogonis was 0.301 6 mg/g, and in Rootlet ophiopogonis was 0.359 4 mg/g. The average recovery rate was 100.30% and RSD was 3.14%. Conclusion The polysaccharide content in Rootlet Ophiopogonis was not lower than in Radix Ophiopogonis. It deserves to be more developed and researched.
