Determination of Ginsenoside Rb_1 in Compound Chanshu Granule by RP-HPLC
- VernacularTitle:RP-HPLC法测定复方产舒颗粒中人参皂苷Rb_1的含量
- Author:
Jian FENG
;
Yanfeng WANG
;
Ying LIU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Chanshu Granule;
Ginsenoside Rb1
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of Ginsenoside Rb1 by RP-HPLC. Methods HPLC was employed with a column of Diamonsil ODS-C18 and a mobile phase of Acetonitrile-H2O (29∶71), the detection wavelength at 203 nm and the column temperature at 25 ℃. Results The linear correlation is available between 0.776 and 7.760 ?g/mL of Ginsenoside Rb1, and the correlation coefficient was 0.999 99, the average recovery was 99.69% and RSD was 2.04% (n=6). Conclusion The method is simple and accurate, and applicable to control the quality of Chanshu Granule.
