Analysis of DNA sequence of Chinese medicinal materials deers and PCR identification of Cervus elaphus and C. nippon
- VernacularTitle:鹿源类中药材DNA序列分析及马鹿和梅花鹿的PCR鉴定
- Author:
Genben BAI
;
Linyuan ZHANG
;
Chunsheng LIU
;
Wei CHENG
;
Daixian CHEN
- Publication Type:Journal Article
- Keywords:
deer;
DNA sequence;
PCR identification
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the animal drug of Cervus elaphs and C. nippon from origin of deers. Methods To extract DNA from deer blood and hairy antler of 11 species of deers such as C. elaphus, C. nippon and so on, and to gain the mitochondrial 12S rRNA gene fragment using the general primers of L1091 and H1478. Based on the sequence multialinement of 11 species deers above gene fragments, designing the couples of special difference primers and identifying C. elaphs and C. nippon. Results 12S rRNA Gene fragments can distinguish different deers well. The couple of primers (EP-1/H1478 and EP-2/H1478) PCR can effectively identify C. elaphus and C. nippon. Conclusion Special primer PCR is suitable for the identification of valuable Chinese medicinal materials, such as C. elaphus and C. nippon.
