BCL-2 METHYLATION IN THE DEVELOPMENT OF BREAST CANCER AND THE RELATIONSHIP BETWEEN BCL-2 METHYLATION AND EXPRESSION
- VernacularTitle:乳腺癌发展过程中的bcl-2甲基化及其与蛋白表达的关系
- Author:
Hongzhi LI
;
Jiazhen WANG
;
Ying PENG
;
Dong LI
- Publication Type:Journal Article
- Keywords:
Beast cancer;
Atypical hyperplasia;
DNA methylation;
Polymerase chain reaction;
Immunohistochemistry
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish MSP (methylation-specific PCR) method for detecting bcl-2 gene, to study bcl-2 methylation in the development of breast cancer and the relationship between bcl-2 methylation and expression. Methods Designing the MSP primer of bcl-2 gene, the methylations in CpG island of bcl-2 gene 5'-regulatory region in 30 cases of normal, 25 cases of atypical hyperplasia(precancerous condition), 40 cases of lymphnode(-)(low-stage) and 45 cases of lymphnode(+)(high-stage) breast tissue were detected by using MSP. The expressions of bcl-2 were detected by using SP immunohistochemical technique. Results The positive rates of bcl-2 methylation in normal, atypical hyperplasia, lymphnode(-) and lymphnode(+) breast tissue were 10.0%,32.0%,40.0% and 53.3% respectively. The methylation of bcl-2 was increased and the expression of bcl-2 was decreased in the development of breast cancer(P
