Effect of Ligustrazine on the Intracellular Translocation of Smad Protein in HSC-T6 Cell Line
- VernacularTitle:川芎嗪对HSC-T6细胞Smad蛋白细胞内转位的影响
- Author:
Bo TAN
;
Jianping SONG
;
Fengxue ZHANG
;
Ni LIU
;
Caijun LI
;
Ruling LU
;
Xingbo GUO
- Publication Type:Journal Article
- Keywords:
Ligustrazine;
Hepatic fibrosis;
HSC;
Smad protein;
Translocation
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ligustrazine on the intracellular translocation of Smad protein in HSC-T6 cell line.Methods HSC-T6 cell was cultured with ligustrazine at the dose of 10-5 mol/L in the culturing dish for 2 hours.After the culturing,the translocation of Smad-2 and Smad-4 proteins was detected by immunofluorescence assay.Results There was no evidence of the translocation of Smad-2 and Smad-4 proteins in HSC-T6 cell after the culturing.Conclusion Ligustrazine can block the signal pathway of TGF-?/Smad,which may be one of its important mechanisms of inhibiting the proliferation of HSC-TS cell.
