Determination of Oridonin in Rabdosia nervosa by HPLC
- VernacularTitle:显脉香茶菜中冬凌草甲素的HPLC测定
- Author:
Honghua CUI
;
Shenglin LIANG
;
Ruifang ZHANG
;
Hongfei CAI
;
Youheng GAO
- Publication Type:Journal Article
- Keywords:
Rabdosia nervosa;
Oridonin;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for the determination of oridonin in the leaves and stems of Rabdosia nervosa.Methods All of reference substances and samples were separated with the mobile phase of methanol:water (45 ∶55) under isocratic elution for 20 min on a Kromasil C18 reversed-phase column(250 mm?4.0 mm,5 ?m),the flow rate was 1.0 mL?min-1,the detection wavelength was 235 nm,and the column temperature was 30 ℃.Results The content of oridonin was 0.047 mg/g in stems and 0.791 mg/g in leaves,the content in stems being about one seventeenth of that in leaves.The average recovery of oridonin was 98.33 %.Conclusion This method is simple,sensitive,accurate and suitable for quantitative determination of oridonin in Rabdosia nervosa.
