Determination of Quercetin in Semen Hoveniae by RP-HPLC
- VernacularTitle:HPLC法测定枳椇子中槲皮素的含量
- Author:
Fenyun SONG
;
Dezhi ZHANG
;
Zhaojian ZHONG
;
Zhiqing PAN
- Publication Type:Journal Article
- Keywords:
Semen Hoveniae;
Quercetin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of quercetin in Semen Hoveniae.Methods A RP-HPLC method was established.The chromatographic column was Synergi 4u Fusion-RP 80.The mobile phase was acetonitrile-0.5 %phosphoric acid(33 ∶67).The flow rate was 1.0 mL?min-1,the detection wavelength was at 360 nm,and the column temperature was 30 ℃.Results The average recovery of quercetin was 98.6 %,and RSD was 2.39 %(n=6).Conclusion The method is effective,simple and accurate,and can be used to determine the content of quercetin in Semen Hoveniae.
