Determination of ferulic acid and gastrodine in Dachuanxiong Hydrochloride for Injection by HPLC
- VernacularTitle:HPLC测定注射用大川芎(冻干)中阿魏酸和天麻素的含量
- Author:
Yanli XU
;
Tianfu LIU
;
Zhongyan WANG
;
Fengkui MO
;
Yuxia HE
;
Yongbo YAN
- Publication Type:Journal Article
- Keywords:
HPLC;
Dachuanxiong Hydrochloride for Injection.;
ferulic acid;
gastrodine
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To set up a method for determining ferulic acid and gastrodine in Dachuanxiong Hydrochloride for Injection(Rhizoma Chuanxiong, Rhizoma Gastrodiae). METHODS: HPLC conditions consisted of ODS column, methanol-water-acetic acid (30 ∶68 ∶2) and water-acetic acid (100 ∶1) as mobile phases, detection wavelengths were at 320 nm and 270 nm. RESULTS: For ferulic acid, the linear range was within 0.031 6 - 0.505 6 ?g and the average recovery was 98.43% with RSD= 1.52% . For gastrodine, the linear range was within 0.442 - 3.536 ?g and the average recovery was 98.15% with RSD= 1.68% . CONCLUSION: The method proves to be simple, precise and reproduciable and is suitable for the use of quantitative control of Dachuanxiong Hydrochloride for Injection.
