Effects of Shenghua Decoction on the expression of adhesion molecules in vascular endothelial cells of blood-stasis rats
- VernacularTitle:生化汤对血瘀证大鼠血管内皮细胞黏附分子表达的影响
- Author:
Xiaoqin HU
;
Liguo CHEN
;
Yuan QU
- Publication Type:Journal Article
- Keywords:
blood-stasis;
vascular endothelial cells;
Shenghua Decoction;
adhesion molecules;
immunohistochemistry;
RT-PCR;
rat
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of Shenghua Decoction on the expression of adhesion molecules in vascular endothelial cells (VECs) of blood-stasis rats. METHODS: The expression of VCAM-1, ICAM-1, PECAM-1, iNOS and their corresponding mRNA in VECs were assayed by immunohistochemistry, image analysis and RT-PCR under the function of different Shenghua Decoction doses (high, middle, low). RESULTS: Immunohistochemistry and image analysis indicated: the expression of VCAM-1, ICAM-1, PECAM-1 and iNOS were higher in the model group than in the control group, Shenghua Decoction could decrease the expression of VCAM-1, ICAM-1, PECAM-1 and iNOS in the model group, moreover as the dose decreased, the expression of these molecules increase, it was of dose-effect relation. CONCLUSION: Shenghua Decoction can downregulate the expression of adhesion molecules in vascular endothelial cells of blood-stasis rats, and the dose-effect relation is obvious.
