Preparation of chondroitin sulfate with different molecular weight and antioxitation capacity
- VernacularTitle:不同分子量硫酸软骨素的制备和抗氧化活性探讨
- Author:
Shuangli XIONG
;
Zhengyu JIN
- Publication Type:Journal Article
- Keywords:
chondroitin sulfate;
isolation and purification;
antioxidation
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore in vitro antioxidan activity of ChS extracted by two enzyme hydrolyses. METHODS: Papain,sodium hydroxide-trypsin were used to extract chondroint sulfate, respectively,DEAE-52 and DEAE-Sepharose FF ion exchange column chromatography were used to isolate and purify crude polysaccharide.RESULTS: The results showed that extraction rate of papain,sodium hydroxide-trypsin for chS was (39.51? 0.89 )%,and (21.23?1.65)%,respectively.DEAE-Sepharose FF was better than DEAE-52 for the isolation and purification of crude polysaccharide,two components were separated and purified from crude polysaccharide extracted by two extraction methods.Four components were separated and purified from crude polysaccharide extracted by papain on the DEAE-Sepharose FF.Three components were obtained by sodium hydroxide-trypsin.Molecular weight of ChS extracted by papain,sodium hydroxide-trypsin was 43569 Da,25773 Da,respectively.The DPPH?, ?OHand O~-_2? scavenging activity of the former was better than those of the latter. CONCLUSION: The study shows that the function of the ChS is probably related to extration mode,molecular weight and conformation.
