Cordyceps Sinensis cDNA Library Construction and Indentification
- VernacularTitle:冬虫夏草cDNA文库的构建和鉴定
- Author:
Yannan LIU
;
Yongsan JIN
;
Zhen LIU
- Publication Type:Journal Article
- Keywords:
Chinese medicinal herb;
Cordyceps sinensis;
cDNA library;
construction
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a cDNA library from Hepialus armoricanus parasitized by Cordyceps sinensi. Methods Total RNA was extracted from Hepialus armoricanus parasitized by Cordyceps sinensi and mRNA was purified, cDNA was synthesized by reverse transcription, and cDNA fragments larger than 1kb were ligated with dephosphorylated arms of pBluescriptⅡ SK(+) XR vectors. The recombinant vectors were eletroporated into DH10B.The size of cDNA inserts and the diversity of library were detected by PCR. Results The cDNA library of stroma contained 1.28?106 recombine clone,that of mycelium was 1.0?106 recombine clone. The rate of recombination of stroma was 87.5%,that of mycelium was 68.75%. The insert size ranged from 0.5 to 2 kb. Conclusion The cDNA library Hepialus armoricanus parasitized by Cordyceps sinensi has been constructed successfully.
