Content Determination of Ferulic Acid in Baidianfeng Pill by HPLC
- VernacularTitle:HPLC法测定白癜风丸中阿魏酸的含量
- Author:
Xingui PAN
;
Yang WANG
;
Xiaodong FENG
- Publication Type:Journal Article
- Keywords:
Baidianfeng Pill;
Ferulic acid;
RP-HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determining the ferulic acid in Baidianfeng Pill. Method ODS column (4.6 mm?250 mm, 5 ?m) was used, the mobile phase consisted of methanol-1% acetic acid aqueous solution(1∶1). The flow rate was 1.0 mL/min and column temperature was at 30 ℃. The detecting wave length was at 320 nm. Results The method was linear with the range of 11.20~84.0 ?g/mL for ferulic acid (r =0.9 998). The average recovery was 98.45%. Conclusion The method is simple, rapid and accurate. It can be used for quantitative deternination of ferulic acid in Baidianfeng Pill.