Determination of Karanjin in Radix of Fofdia Caulifora by Thin Layer Chromatograph- Fluorescent Method
- VernacularTitle:薄层色谱-荧光分析法测定水罗伞药材中水黄皮素含量的研究
- Author:
Dongai YANG
;
Lifang YANG
;
Shaomei MA
- Publication Type:Journal Article
- Keywords:
Radix of Fofdia caulifora;
karanjin;
TLC - Fluorescent method
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a thin layer Chromatograph-Fluores cent method for the determination of karanjin in Radix of Fofdia caulifora. Methods Anhydrous-EtOH is solution, thin-sheet is silica gel, expanding solution is Benzene-EtoAc (8.5∶1.5), with excitation wavelength at 355 nm and emission wavelength at 428 nm. Results The average recovery of karanjin was 99.14% and RSD was 1.94% (n =5). There is a good linear relationship within the range of 0.138～0.69 ?g of karanjin. Conclusion The method is convenient, sensitive, accurate and reproducible, and can be used the quality control of the Radix of Fofdia caulifora.
