Isolation of Rat Marrow Mesenchymal Stem Cells and Effect of Conditioned Medium on Its Proliferation

VernacularTitle:骨髓间充质干细胞分离及条件培养基在其培养扩增中的作用
Author: Jie ZHENG ; Peixun WANG ; Lian ZHOU
Publication Type:Journal Article
Keywords: MESENCHYMAL STEM CELLS/isolation & purification; CELL CULTURE
From: Journal of Guangzhou University of Traditional Chinese Medicine 2000;0(04):-
CountryChina
Language:Chinese
Abstract: 【Objective】To establish a simple,rapid and practical method for the in-vitro isolation,purification and proliferation of rat marrow mesenchymal stem cells(MSCs).【Methods】Rat MSCs were isolated and purified by differential adhesion method.The effects of conditioned medium on the morphologic characteristics and growth of rat MSCs were observed.【Results】Rat MSCs were effectively purified by differential adhesion method,and grew well with good proliferation and normal morphology in conditioned medium.【Conclusion】Differential adhesion method and conditioned medium are effective for the purification and culture of rat MSCs.