Amplification of 5′cDNA end of human augmenter of liver regeneration by 5′-RACE technique and analysis of sequence
- VernacularTitle:利用5′-RACE技术进行人肝再生增强因子相互作用肽cDNA5′末端快速扩增及序列分析
- Author:
Jianchuan DENG
- Publication Type:Journal Article
- Keywords:
5'-RACE;
hALR;
Interacting peptide;
cDNA
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To amplify the 5′ cDNA end of human augmenter of liver regeneration(hALR) by 5′-RACE technique and to analyze the sequence.Methods: 5′ cDNA end of hALR was rapidly amplified by following the instructions of 5′-RACE kit(Takara).The acquired sequence was analyzed by bioinformatic tool.Results: 5′-RACE product with 487bp was successfully obtained,which included extending sequence with 275bp.Homology analysis by BLAST showed highly homologous with human citron kinase.Conclusions: 5′-RACE method can be used to amplify the 5′ end of partially known cDNA sequence.Citron kinase can interact with hALR.
