Activation of extracelluar signal-regulated kinase pathway inhibits cytotoxic effect of SW480 cell in response to 5-aminolevulinic acid-based photodynamic therapy
- VernacularTitle:细胞外信号调节激酶通路激活抑制?氨基酮戊酸-光动力疗法对SW480细胞的细胞毒作用
- Author:
Jianghua ZHENG
- Publication Type:Journal Article
- Keywords:
ALA;
PDT;
SW480;
ERK pathway
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate extracelluar signal-regulated kinase(ERK) pathway and cytotoxic effect after block of this pathway after 5-aminolevulinic acid(ALA)-based photodynamic therapy(PDT) on SW480 cell.Methods: SW480 was divided into control group,light group,ALA group,ALA-PDT group and PD98059 group.Western blot was used to detect the expression of protein products and phosphorylation protein products of MEK and ERK1/2.Optical density value and survival rate of each group were obtained at different time by MTT.Results: ERK pathway of SW480 cell was activated and block of this pathway increased cytotoxic effect of SW480 cell after ALA-PDT.Conclusions: Activation of ERK pathway protects SW480 cell from ALA-PDT.Block of ERK pathway at different levels may become a new target,which will enhance cytotoxic effect of ALA-PDT on colon carcinoma.
