The mechanism of PTEN down-regulation on neuron intracellular free calcium and apoptosis
- VernacularTitle:PTEN表达下调对神经元胞内游离钙离子和神经元凋亡的研究
- Author:
Lixue CHEN
- Publication Type:Journal Article
- Keywords:
PTEN;
shRNAs;
Ca2+;
Apoptosis;
Neuron culture
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the regulation of PTEN on apoptosis and the active roles in blocking Ca2+ influx and neuroprotection.Methods: In the OGD model with anaerobic gas mixture and deoxygenated,glucose-free extracellular solution,shRNAspten-GFP and unrelated control plasmid PshGFP transfection were done respectively.Intracellular free calcium concentration were measured with Fura-2 AM fluorescent indicator,the expression of PTEN were analyzed by immunoblotting(blot) and neuron apoptosis were detected by AO/EB and Flow Cytometry.Results: Apoptosis rate and calcium concentration after OGD was significantly higher than that of normal group(P
