The study of killing effect by T-lymphocytes after activiated by dendritic cells loaded with RCC 786-0 cell line freeze thawing antigen in vitro
- VernacularTitle:肾癌冻融抗原致敏树突状细胞介导的CTL对肾癌786-0细胞株的体外杀伤作用研究
- Author:
Zili HU
- Publication Type:Journal Article
- Keywords:
Renal carcinoma cell;
Dendritic cells;
Antitumor immune;
CTL
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the immune responses of cytotoxic T-lymphocytes against Renal carcinoma cell 786-0(RCC) after activiated by dendritic cells(DCs) loaded with RCC freeze thawing antigen.Methods:DCs were obtained by cultured plastic-adherent monocytes isolated from health human peripheral blood with granulocyte-monocyte colony-stimulating factor(GM-CSF) and interleukin-4(IL-4) and Tumor necrosis factor(TNF) for 9 days.Host lymphocytes were stimulated with freeze thawing antigen of RCC 786-0 under the culture medium containing interleukin-2(IL-2) for 5 day.Killing activity and cytokine release were measured by MTT assay and ELISA.Results:The immune response of CTL activiated by DCs loaded with tumor soluble antigen was demonstrated by the following facts:(1)DCs loaded with tumor antigen could induce the growth of CTL.(2)The cyotoxicity of obtained specific CTL against RCC-7860 was highly enhanced with a significant difference from that of non-specific CTL.(3)The interleukin-12 release/secretion was increased by tumor antigen,which suggest an improved anti-tumor effect.(4)Apoptosis was observed in the RCCs after treated with CTL obtained.Conclution: These findings indicate that specific CTL induced by DCs sensibilized by RCC freeze thawing antigen exerts a remarkable killing activity on RCC 786-0.It is suggestd that DCs antitumor vaccines poses a clinically useful prospect with RCC.
