Effect of carthamus tinctorius L.injection on caspase-3,bcl-2 expression related to renal ischemia/reperfusion injury in rats
- VernacularTitle:红花注射液对大鼠肾缺血再灌注损伤Caspase-3和bcl-2表达的影响
- Author:
Fei GAO
- Publication Type:Journal Article
- Keywords:
Carthamus tinctorius injection;
Ischemia/reperfusion;
Apoptosis;
Caspase-3;
Bcl-2
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
Objective: To investigate the effect of carthamus tinctorius injection on expression of caspase-3 and bcl-2 after renal ischemia/reperfusion injury.Methods: Seventy-five SD rats were randomly divided into three groups(n=25,in each):normal control group,ischemia/reperfusion group and treatment group.After the renal ischemia/reperfusion injury model was established,the serum creatinine、the urine osmotic pressure and the blood osmotic pressure were measured to evaluate the change of renal function;the apoptosis rate in ischemia/reperfusion renal tissure were measured by TUNEL method;the caspase-3 and bcl-2 concentration were measured by immunohistochemistry.Results:The renal function got worse,and the apoptosis rate,bcl-2 and caspase-3 concentration were significantly higher in ischemia/reperfusion group than those in normal control group.As compared with ischemia/reperfusion group,the renal function was improved,the apoptosis rate decreased,bcl-2 expression increased and caspase-3 expression decreased significantly in treatment group.Conclusion:Carthamus tinctorius injection has significant effect on the up-regulation of bcl-2 and down-regulation of caspase-3 expression in renal ischemia/reperfusion injury.
